3C and TEV proteases substrate (Prescission & TEV protease substrate, GST-PC3C)

Introduction

Substrate protein for control cleavage by both 3C (Prescision) and TEV proteases.

Description

The 44 kDa substrate protein is specifically cleaved to 26 kDa and 18 kDa fragments by both TEV and 3C proteases.

Application

Functional Studies, Protein-protein interactions, Gel-shift assay, Pull-down assay, DNA binding and stabilization

Purification method

Affinity chromatography

Formulation

1 mg/ml, 40% glycerol, 50mM Tris, 100mM NaCl. Stable protein, occasional melting of ice packs during shipping does not affect the integrity.

Specificity

3C (Prescission) protease, TEV protease

Storage

-80°C, aliquot to avoid repeated freezing and thawing.