FRET HRV-3C substrate (Prescission fluorescent substrate)

Introduction

Substrate protein for quantitative determination and monitoring of human rhinovirus 3C (HRV-3C, Prescission) protease specific activity based on fluorescent energy transfer (FRET). Suitable for high-throughput applications.

Description

The 60 kDa FRET substrate protein is composed by two fluorescent proteins linked with Caspase-3 recognition sequence LEVLFQ|GP. The substrate is specifically cleaved to fluorescent monomers, which results in quantitative decrease of fluorescent intensity at 580-650 nm (emission). The excitation range of the substrate is 490-515 nm.

Application

Protease activity control and monitoring. High-throughput screening of Prescission (HRV-3C) protease variants. This substrate is manufactured in certified laboratory environment and could be used in GMP certified downstream processes.

Purification method

Affinity chromatography, size exclusion chromatography, desalting.

Formulation

0.5 mg/ml, 10mM Tris pH7,5, 50mM KCl

Specificity

HRV-3C protease recognition sequence.

Storage

-80°C, aliquot to avoid repeated freezing and thawing.