Anti-AHP4 (AHP4@H3, Histidine kinase,)

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Introduction

Monoclonal antibodies against Arabidopsis thaliana phosphotransfer protein 4 (AHP4)

Description

Anti-AHP antibodies are indispensable reagents for scientists focused on unraveling of plant internal clock. Anti-AHP monoclonal antibodies were developed in Central European Institute of Technology (CEITEC), Brno, Czech Republic, as a unique tool for mapping of multistep phosphorelay signaling in A. thaliana. This antibody collection represents the most complex tool for mapping of plant stimuli signal transfer from sensor histidine kinase to nuclear response regulators, via histidine phosphotransfer proteins. AHP antibodies are suitable not only for qualitative investigation of magnesium dependent phosphotransfer relay, but also for quantification of particular sensor histidine kinases.

Application

Affinity Purification, Western Blotting, ELISA, Protein Array, Protein-Protein Interactions, Pull-down Assay. WB working dilution 1:10 000.

Purification method

Affinity chromatogramy on protein G.

Formulation

50 mM Tris-HCl pH 8 with 20 % of glycerol in final concentration 1 mg/ml.

Specificity

Use AHP4@H3 when you are interested in: - young flower-specific expression - thickening of the secondary cell wall of the anther endothecium - in formation of secondary cell walls via the action of AHP4 - in vivo and in vitro detection of AHP4 - new interaction of AHP4 proteins with other - proteins - quantification of protein interactions mediated by AHP4 - sub-cellular localization of AHP4 - detection of splicing variants of AHP4 - analysis of stability of AHP4

Storage

Flash frozen in liquid nitrogen. Store at -80 °C. Avoid repeated freezing and thawing.