Diagnostics using isothermal DNA amplification (LAMP, RT-LAMP)

Protean Ltd. has developed technology for a simple detection of various pathogens and specific DNA and RNA sequences based on the isothermal amplification technique. The technology profits from in-house production of amplification enzymes BstI DNA polymerase and ProRT Reverse Transcriptase and fully complies with CE IVD certification standards. A patent application was filed for the technological solution of this diagnostics.

Major advantages:

Easy on laboratory equipment and staff - it can be done in any laboratory, no PCR cycler required.

Results are available within one hour - with the ability to read results with the naked eye or mobile (also spectrophotometrically).

Huge test throughput - hundreds of samples can be analyzed at once.

Significant cost reduction over traditional PCR assays while maintaining the same sensitivity and specificity.

Test principle

RT-LAMP (reverse transcription and loop-mediated isothermal amplification) allows amplification of nucleic acids with a high specificity, efficiency and speed under isothermal conditions - ie. at constant temperature, achievable in incubators, hybridization ovens or dry blocks. The results are reliably read based on the color change by naked eye.

Necessary material not supplied with the kit

Test sensitivity